Generator
Part:BBa_K624051:Design
Designed by: Yi-Hong Liou Group: iGEM11_NYMU-Taipei (2011-10-05)
pYMB essentials + RBS(trunc.) + LLO + RBS(trunc.) + Invasin + RBS(trunc.) + ECFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2558
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3555
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 4172 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
LLO: PCR amplification from the genomic DNA of Listeria monocytogenes
Inv: PCR amplification from the genomic DNA of Yersinia enterocolitica